电离辐射通过转化生长因子-β-介导的上皮-上皮转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

内容可 ：探讨远红外线否可通过转换成生长因子-β（TGF-β）-介导的上皮-泌尿系统转换 (EMT)来促进骨髓的首当其冲迁往。采行量2Gy(60)Coγ线紫外光源自人类心脏的6种骨髓，记录与EMT具体的变化，这包括分别透过显微镜新技术，氨基酸质印迹方通则，免疫荧光新技术，划痕试验和Transwell小室试验来观察并检查细胞组织形态，EMT标记，首当其冲迁往能力也等。采行酶嗣后免疫吸附通则检查这些骨髓中TGF-β氨基酸低水平，透过特别肽SB431542来评估TGF-β信号通路在远红外线EMT中的作用。经过量为2Gy紫外光的骨髓中实际上间叶细胞的传达，与实为紫外光组相比其上皮标记减少，间叶细胞标记增加，同时其首当其冲转移能力也加强，TGF-β氨基酸低水平也提高。大幅度发现由A549远红外线诱导的EMT可通过对TGF-β信号抑制发生完胜。这些结果表明TGF-β介导的EMT在远红外线诱导加强骨髓首当其冲转移能力也中起着关键作用。

出版人： lizexiu